6MOJ

Dimeric DARPin A_angle_R5 complex with EpoR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97741
Spacegroup name	I 41 2 2
Unit cell lengths	130.418, 130.418, 293.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.110 - 2.431
R-factor	0.2123
Rwork	0.210
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predicted model 1ern
RMSD bond length	0.003
RMSD bond angle	0.603
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.739	2.730
High resolution limit [Å]	2.431	2.431
Rmerge	0.273	2.759
Rpim	0.054	0.551
Number of reflections	25096	1280
<I/σ(I)>	11.04	0.46
Completeness [%]	52.4	9.2
Redundancy	26	25.7
CC(1/2)	0.999	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	0.2 M sodium potassium tartrate, 0.1 M bis-tris propane pH 8.5, 20% PEG 3350, 30% glycerol as cryoprotectant