6MOH

Dimeric DARPin C_R3 complex with EpoR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999924
Spacegroup name	P 43 21 2
Unit cell lengths	96.193, 96.193, 286.401
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.019 - 3.200
R-factor	0.2383
Rwork	0.236
R-free	0.27670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mog 1ern
RMSD bond length	0.002
RMSD bond angle	0.610
Data reduction software	XDS (June 1, 2017)
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.020	3.315
High resolution limit [Å]	3.200	3.200
Rmerge	0.889	5.596
Rpim	0.281	1.771
Number of reflections	18878	944
<I/σ(I)>	3.07	1.4
Completeness [%]	80.0	17.52
Redundancy	11.2	11.3
CC(1/2)	0.965	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	295	0.1 M HEPES pH 7.3, 1.4 M sodium potassium phosphate, 24% glycerol cryoprotectant