6MOE

Monomeric DARPin E2 complex with EpoR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033202
Spacegroup name	P 21 21 21
Unit cell lengths	69.250, 122.160, 132.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.765 - 2.091
R-factor	0.2047
Rwork	0.204
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ern
RMSD bond length	0.003
RMSD bond angle	0.633
Data reduction software	XDS (Jun 1, 2017)
Data scaling software	XDS (Jun 1, 2017)
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.765	2.166
High resolution limit [Å]	2.091	2.091
Rmerge	0.132	2.671
Rpim	0.037	0.739
Number of reflections	67078	6559
<I/σ(I)>	12.15	0.5
Completeness [%]	99.4	96.63
Redundancy	13.6	13.8
CC(1/2)	0.999	0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	295	1 M sodium potassium phosphate pH 5.6 Cryoprotected in 25% ethylene glycol