6MNH
ULK1 Unc-51 like autophagy activating kinase in complex with inhibitor BTC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-13 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 101.984, 44.884, 58.022 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.140 - 1.730 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22780 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.191 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.140 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.065 | 0.425 |
| Number of reflections | 28537 | 4090 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 281 | 31% PEG 4K, 200mM calcium chloride, 100mM bis tris propane pH 8.5 |
