6MM6
Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 phosphorylation domain (2699-2904)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.451, 74.944, 88.690 |
| Unit cell angles | 105.51, 90.14, 94.39 |
Refinement procedure
| Resolution | 34.870 - 2.390 |
| R-factor | 0.2254 |
| Rwork | 0.224 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mm5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.416 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 2.460 |
| High resolution limit [Å] | 2.390 | 5.470 | 2.390 |
| Rmerge | 0.119 | 0.038 | 0.735 |
| Rmeas | 0.161 | 0.051 | 0.998 |
| Rpim | 0.108 | 0.034 | 0.670 |
| Total number of observations | 123858 | ||
| Number of reflections | 56090 | 4692 | 4655 |
| <I/σ(I)> | 5.2 | ||
| Completeness [%] | 98.5 | 98.6 | 97.8 |
| Redundancy | 2.2 | 2.2 | 2.2 |
| CC(1/2) | 0.996 | 0.458 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.05M HEPES, 0.05 M KCl, 0.01M MgCl2, 15% (w/v) PEG 6K, and 25% (v/v) ethylene glycol |
