6MFS
Mouse talin1 residues 1-138 fused to residues 169-400 in complex with phosphatidylinositol 4,5-bisphosphate (PIP2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 39.686, 73.055, 162.342 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.410 - 2.850 |
R-factor | 0.239 |
Rwork | 0.237 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ivf |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | XDS (Nov 1 2016) |
Data scaling software | Aimless |
Phasing software | PHASER (0.5.32) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.590 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 11685 | |
<I/σ(I)> | 11 | 3.3 |
Completeness [%] | 93.9 | 99.8 |
Redundancy | 6 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 18-25% PEG 3350, 200 mM Ammonium citrate pH 7.0 |