6MF2
Improved Model of Human Coagulation Factor VIII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 134.569, 134.569, 359.496 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.365 - 3.609 |
R-factor | 0.253947279805 |
Rwork | 0.252 |
R-free | 0.28356 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.149 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.365 | 3.738 |
High resolution limit [Å] | 3.609 | 3.609 |
Number of reflections | 31887 | |
<I/σ(I)> | 0.7 | |
Completeness [%] | 81.7 | |
Redundancy | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 8-10% PEG8000, 0.1 M imidazole, 100-300 mM sodium chloride |