6MEV
Structure of JMJD6 bound to Mono-Methyl Arginine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2016-10-13 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.547, 141.906, 149.263 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 64.000 - 2.600 |
| R-factor | 0.2557 |
| Rwork | 0.229 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.000 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.220 | 1.542 |
| Number of reflections | 127739 | |
| <I/σ(I)> | 8.36 | |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281.15 | 0.1M sodium citrate pH 5.6 1.0M ammonium phosphate monobasic |
