6MEK
Crystal structure of Hepatitis C virus envelope glycoprotein E2 core in complex with human antibodies HEPC3 and HEPC46
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.399, 105.589, 337.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.967 - 3.100 |
R-factor | 0.2265 |
Rwork | 0.223 |
R-free | 0.28990 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 89.490 | 89.490 | 2.980 |
High resolution limit [Å] | 2.900 | 12.970 | 2.900 |
Rmerge | 0.160 | 0.048 | 1.632 |
Rmeas | 0.168 | 0.051 | 1.745 |
Rpim | 0.052 | 0.015 | 0.593 |
Total number of observations | 617678 | ||
Number of reflections | 60807 | 807 | 4367 |
<I/σ(I)> | 10.7 | ||
Completeness [%] | 99.9 | 99.8 | 99.6 |
Redundancy | 10.2 | 10.6 | 8.2 |
CC(1/2) | 0.997 | 0.999 | 0.411 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.02 M Nickel(II) chloride hexahydrate, 0.02 M magnesium chloride hexahydrate, 0.02 M cadmium chloride hydrate, 0.1 M sodium acetate trihydrate pH 4.5, 24% PEG 2,000 |