6MEH

Crystal structure of broadly neutralizing antibody HEPC74 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	66.309, 69.770, 168.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.764 - 1.990
R-factor	0.1883
Rwork	0.186
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mwf 6mee
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	84.290	84.290	2.040
High resolution limit [Å]	1.990	9.110	1.990
Rmerge	0.112	0.053	1.138
Rmeas	0.123	0.059	1.337
Rpim	0.050	0.024	0.664
Total number of observations	317427
Number of reflections	53916	675	3261
<I/σ(I)>	7.3
Completeness [%]	98.7	99.4	87
Redundancy	5.9	5.6	4.9
CC(1/2)	0.959	0.995	0.026

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	1% tryptone, 0.001 M sodium azide, 0.05 M HEPES sodium pH 7.0, 20% PEG 3,350)