6MCO
Crystal structure of the B41 SOSIP.664 Env trimer with PGT124 and 35O22 Fabs, in P23 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03315 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 212.326, 212.326, 212.326 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.046 - 3.526 |
| R-factor | 0.307 |
| Rwork | 0.306 |
| R-free | 0.32230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r26 5cez |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.909 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.046 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rpim | 0.100 | |
| Number of reflections | 39580 | 3932 |
| <I/σ(I)> | 10.1 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 37.5 | 30.6 |
| CC(1/2) | 0.840 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1M HEPES (pH 7.0), 8% PEG8000 |
