6MAK
HBO1 is required for the maintenance of leukaemia stem cells
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.953736 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 87.628, 39.026, 107.765 |
Unit cell angles | 90.00, 99.59, 90.00 |
Refinement procedure
Resolution | 43.200 - 2.130 |
R-factor | 0.21629 |
Rwork | 0.214 |
R-free | 0.26434 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.204 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.200 | 2.190 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.070 | 0.748 |
Rpim | 0.029 | 0.310 |
Number of reflections | 19938 | 1558 |
<I/σ(I)> | 13.1 | 2.6 |
Completeness [%] | 98.0 | 94.2 |
Redundancy | 6.9 | 6.6 |
CC(1/2) | 0.998 | 0.882 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein at 2.5 mg/mL was added in equal volume ratio with reservoir, 200 nL plus 200 nL. The reservoir consisted of 22% PEG MME 2000 plus 57 mM diammonium tartrate. Plates were stored at 20 C. |