6M4V
Crystal structure of MBP fused split FKBP in complex with rapamycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-11-20 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 107.026, 48.144, 108.730 |
Unit cell angles | 90.00, 106.52, 90.00 |
Refinement procedure
Resolution | 48.140 - 2.920 |
R-factor | 0.21298 |
Rwork | 0.208 |
R-free | 0.29762 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fap |
RMSD bond length | 0.005 |
RMSD bond angle | 1.390 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.140 | 3.100 |
High resolution limit [Å] | 2.920 | 2.920 |
Number of reflections | 23633 | 3775 |
<I/σ(I)> | 7.4 | |
Completeness [%] | 100.0 | |
Redundancy | 3.8 | |
CC(1/2) | 0.995 | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Na-HEPES buffer (pH 7.5), 20% (w/v) PEG 8000 |