6M4U
Crystal structure of FKBP-FRB T2098L mutant in complex with rapamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.034, 67.419, 69.793 |
| Unit cell angles | 90.00, 107.20, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.200 |
| R-factor | 0.20089 |
| Rwork | 0.198 |
| R-free | 0.25766 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fap |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.157 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.450 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 24948 | 2223 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 96.6 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.995 | 0.849 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM cacodylic acid buffer (pH 6.5), 350 mM zinc acetate and 8% (w/v) isopropanol |
