6M1K
USP7 in complex with a novel inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 273 |
Detector technology | PIXEL |
Collection date | 2019-11-13 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.065, 69.200, 76.450 |
Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
Resolution | 41.180 - 2.255 |
R-factor | 0.2351 |
Rwork | 0.233 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n9t |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3246) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.180 | 2.336 |
High resolution limit [Å] | 2.255 | 2.255 |
Rmerge | 0.075 | |
Rmeas | 0.137 | |
Rpim | 0.074 | |
Number of reflections | 35627 | 3451 |
<I/σ(I)> | 21.3 | |
Completeness [%] | 95.1 | |
Redundancy | 6.3 | |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M Tris (hydroxymethyl)aminomethane hydrochloride, pH 7.0, 20% (v/v) PEG 1000 |