6LR6

The crystal structure of human cytoplasmic LRS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97854
Spacegroup name	C 2 2 21
Unit cell lengths	88.854, 95.798, 682.388
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.412 - 3.009
R-factor	0.2135
Rwork	0.211
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wz2 2wfd
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.116	0.093	0.539
Rmeas	0.131	0.107	0.610
Rpim	0.060	0.051	0.277
Total number of observations	185795
Number of reflections	45642	5070	4305
<I/σ(I)>	6.4
Completeness [%]	77.7	81.5	74.2
Redundancy	4.1	3.9	3.9
CC(1/2)		0.853	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	20% (w/v) PEG 6000, 100 mM Tris-Cl, pH 8.0, and 200 mM Lithium chloride