6LN2

Crystal structure of full length human GLP1 receptor in complex with Fab fragment (Fab7F38)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	63.040, 64.660, 322.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.200
R-factor	0.215
Rwork	0.213
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nx2
RMSD bond length	0.010
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.700	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.116	0.626
Number of reflections	22019	3109
<I/σ(I)>	6.5	1.8
Completeness [%]	97.8	96.7
Redundancy	4.8
CC(1/2)	0.998	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	200-300 mM Ammonium formate, 36% PEG 400, 5%-10% (w/v) Guanidine hydrochloride