6LKQ
The Structural Basis for Inhibition of Ribosomal Translocation by Viomycin
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-04-02 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.03318 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 257.600, 312.900, 328.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 3.100 |
Rwork | 0.210 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3F1E. 3F1F |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.6) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 50.000 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.200 | |
Number of reflections | 433307 | 433307 |
<I/σ(I)> | 1.7 | |
Completeness [%] | 100.0 | |
Redundancy | 7 | |
CC(1/2) | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | Tris Ac PH.7.0, 25-35 mM KCL, 6.1% PEG 20000, 1% glycerol, 50mM sucrose |