6LK5
MLKL mutant - T357ES358D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-09 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.739, 74.848, 127.388 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.450 - 2.500 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m67 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.256 |
| Data reduction software | SCALEPACK |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.149 | 0.062 | 1.155 |
| Rmeas | 0.156 | 0.065 | 1.206 |
| Rpim | 0.044 | 0.019 | 0.343 |
| Total number of observations | 294032 | ||
| Number of reflections | 12272 | 1253 | 1142 |
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 100.0 | 99.1 | 100 |
| Redundancy | 12.7 | 12.5 | 11.9 |
| CC(1/2) | 0.997 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5%(v/v) 2-Methyl-2,4-pentanediol, 0.1M HEPES(pH7.5), 10%(w/v) PEG 10000 |
