6LE5
Crystal structure of the mitochondrial calcium uptake 1 and 2 heterodimer (MICU1-MICU2 heterodimer) in an apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-23 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.970, 173.720, 148.000 |
Unit cell angles | 90.00, 93.88, 90.00 |
Refinement procedure
Resolution | 50.010 - 3.100 |
R-factor | 0.29467 |
Rwork | 0.293 |
R-free | 0.33162 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.380 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.860 | 3.180 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 57570 | 4480 |
<I/σ(I)> | 6.2 | |
Completeness [%] | 100.0 | |
Redundancy | 7.3 | |
CC(1/2) | 0.992 | 0.258 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG3350 0.2 M Ammonium citrate tribasic (pH 7.5) |