6LDM
Structural basis of G-quadruplex DNA recognition by the yeast telomeric protein Rap1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2015-09-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.680, 70.200, 64.850 |
Unit cell angles | 90.00, 97.72, 90.00 |
Refinement procedure
Resolution | 35.090 - 2.400 |
R-factor | 0.2043 |
Rwork | 0.203 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ign |
RMSD bond length | 0.009 |
RMSD bond angle | 0.890 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 64.262 | 32.131 | 2.530 |
High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
Rmerge | 0.030 | 0.406 | |
Rmeas | 0.068 | 0.037 | 0.515 |
Rpim | 0.040 | 0.021 | 0.312 |
Total number of observations | 1439 | 5258 | |
Number of reflections | 14665 | 480 | 2134 |
<I/σ(I)> | 8.7 | 19.4 | 2 |
Completeness [%] | 99.1 | 97.5 | 99.5 |
Redundancy | 2.7 | 3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.1M Sodium citrate tribasic dihydrate pH 5.6, 10% isopropanol, 14% PEG 4000, 10% of glycerol |