6LD5
Zika NS5 polymerase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-24 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.470, 84.590, 69.150 |
| Unit cell angles | 90.00, 113.90, 90.00 |
Refinement procedure
| Resolution | 34.180 - 1.940 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tfr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.180 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.136 | 0.543 |
| Number of reflections | 46894 | 4079 |
| <I/σ(I)> | 5.89 | |
| Completeness [%] | 95.9 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.981 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 10% PEG 6000, 0.2M HEPES pH 7.4 |
