6L4O

Crystal structure of API5-FGF2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-16
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	46.862, 76.523, 208.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.630 - 2.600
R-factor	0.226
Rwork	0.224
R-free	0.26150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u0r 1bas
RMSD bond length	0.001
RMSD bond angle	0.386
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.105	0.375
Number of reflections	22727	22727
<I/σ(I)>	15.7
Completeness [%]	94.5
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100mM Na-HEPES (pH 7.5), 100mM potassium chloride, 10% (v/v) polyethylene glycol (PEG) 6000