6KX0
Crystal structure of SN-101 mAb non-liganded form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.860, 40.100, 67.780 |
| Unit cell angles | 90.00, 99.83, 90.00 |
Refinement procedure
| Resolution | 39.378 - 2.404 |
| R-factor | 0.2278 |
| Rwork | 0.225 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pz5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.626 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.101 | 0.663 |
| Number of reflections | 16818 | 2665 |
| <I/σ(I)> | 12.78 | |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 4.99 | 5.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 300-350 mM NaSCN 20-30% PEG3350 |
