6KUX
Crystal structures of the alpha2A adrenergic receptor in complex with an antagonist RSC.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.170, 71.790, 284.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.385 - 2.700 |
R-factor | 0.252 |
Rwork | 0.251 |
R-free | 0.26690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y02 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.526 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.700 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.089 | 0.487 |
Number of reflections | 18743 | 2155 |
<I/σ(I)> | 9.7 | |
Completeness [%] | 91.4 | 73.4 |
Redundancy | 8.1 | 2.5 |
CC(1/2) | 0.999 | 0.460 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.1M Sodium citrate tribasic dihydrate pH 5.0, 290mM Ammonium chloride, 30% PEG400, 7% glycerol |