6KUW

Crystal structure of human alpha2C adrenergic G protein-coupled receptor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	74.480, 78.740, 190.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.040 - 2.800
R-factor	0.216
Rwork	0.213
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4s0v
RMSD bond length	0.009
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.110	0.721
Rmeas	0.127
Number of reflections	26778	1692
<I/σ(I)>	8.15	1.74
Completeness [%]	94.4	82.1
Redundancy	3.74	3.23
CC(1/2)	0.996	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	sodium phosphate monobasic (0.1M), Hepes pH6.5-6.9 (0.1M), PEG400 24-35%