6KTR
Crystal structure of fibroblast growth factor 19 in complex with Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2018-04-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.968, 104.125, 165.365 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.717 - 2.598 |
R-factor | 0.204167575911 |
Rwork | 0.202 |
R-free | 0.25319 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2p23 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.587 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.730 | 2.690 |
High resolution limit [Å] | 2.590 | 2.600 |
Rmerge | 0.017 | 0.145 |
Rmeas | 0.205 | |
Rpim | 0.017 | 0.145 |
Number of reflections | 45911 | 4486 |
<I/σ(I)> | 3.3 | 3.3 |
Completeness [%] | 99.7 | 99 |
Redundancy | 2 | 2 |
CC(1/2) | 0.997 | 0.952 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293.15 | 0.2M Lithium Sulfate monohydrate, 0.1M Bis-Tris pH6.9, 26% w/v Polyethylene Glycol 3350 |