6KRZ

Crystal structure of the human adiponectin receptor 1 D208A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-15
Detector	RAYONIX MX-225
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	104.589, 119.350, 197.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.900 - 3.050
R-factor	0.2182
Rwork	0.216
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3WXV
RMSD bond length	0.002
RMSD bond angle	0.430
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549+SVN)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.900	47.900	3.230
High resolution limit [Å]	3.048	9.140	3.048
Rmerge	0.260	0.042	1.494
Rmeas	0.284	0.047	1.631
Rpim	0.114	0.020	0.651
Number of reflections	47972	1943	7573
<I/σ(I)>	7
Completeness [%]	99.8	99.2	99
Redundancy	6.1	5.5	6.2
CC(1/2)	0.992	0.998	0.515

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.5	293	PEG 400, tris, lithium acetate