6KP6
The structural study of mutation induced inactivation of Human muscarinic receptor M4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.100, 61.320, 203.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.140 - 3.000 |
R-factor | 0.233 |
Rwork | 0.231 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dsg |
RMSD bond length | 0.008 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.140 | 49.140 | 3.080 |
High resolution limit [Å] | 3.000 | 13.420 | 3.000 |
Rmerge | 0.116 | 0.062 | 0.586 |
Rmeas | 0.118 | 0.064 | 0.637 |
Total number of observations | 398969 | ||
Number of reflections | 14718 | 201 | 1041 |
<I/σ(I)> | 19.32 | 60.29 | 2.3 |
Completeness [%] | 99.7 | 97.1 | 97 |
Redundancy | 27.108 | 32.393 | 6.028 |
CC(1/2) | 0.996 | 0.995 | 0.379 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7.8 | 293.15 | 300 mM diammonium hydrogen phosphate, 22-26% PEG 300, 0.1M HEPES sodium pH 7.6-8.2 |