6KNZ
Crystal structure of T2R-TTL-KXO1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97777 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.928, 157.059, 182.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.232 - 2.475 |
R-factor | 0.2206 |
Rwork | 0.220 |
R-free | 0.26070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iyz |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.475 | 2.475 |
Number of reflections | 106839 | 5268 |
<I/σ(I)> | 19.5 | |
Completeness [%] | 100.0 | |
Redundancy | 13.4 | |
CC(1/2) | 0.995 | 0.812 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2 |