6KMS

Crystal structure of human N6amt1-Trm112 in complex with SAM (space group I422)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-14
Detector	RAYONIX MX225HE
Wavelength(s)	0.9792
Spacegroup name	I 4 2 2
Unit cell lengths	195.354, 195.354, 246.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.386 - 3.200
R-factor	0.2333
Rwork	0.232
R-free	0.26930
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.374
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.200	6.890	3.200
Rmerge	0.234	0.073	2.203
Rmeas	0.239	0.074	2.244
Rpim	0.044	0.014	0.420
Number of reflections	39672	4180	3900
<I/σ(I)>	26.67
Completeness [%]	100.0	99.6	100
Redundancy	28.8	28.3	28.1
CC(1/2)		0.999	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	1.6M (NH4)2SO4, 0.1M MES, pH 6.5, 10% 1,4-Dioxane