6KM7
The structural basis for the internal interaction in RBBP5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97855 |
Spacegroup name | P 1 |
Unit cell lengths | 57.571, 68.841, 72.788 |
Unit cell angles | 99.24, 99.31, 113.81 |
Refinement procedure
Resolution | 34.772 - 1.801 |
R-factor | 0.1664 |
Rwork | 0.165 |
R-free | 0.19430 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.962 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHARP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.085 | 0.061 | 0.421 |
Rmeas | 0.092 | 0.065 | 0.462 |
Rpim | 0.035 | 0.025 | 0.186 |
Total number of observations | 583704 | ||
Number of reflections | 86928 | 8765 | 8055 |
<I/σ(I)> | 5.4 | ||
Completeness [%] | 96.3 | 97.2 | 89.2 |
Redundancy | 6.7 | 7.2 | 5.5 |
CC(1/2) | 0.987 | 0.911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M HEPES-Na, pH 7.5, 2% w/v PEG 400, 2.0 M ammonium sulfate |