6KDP

Crystal structure of human DNMT3B-DNMT3L complex (II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	110
Detector technology	CCD
Collection date	2018-10-19
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	72.105, 234.066, 230.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.804 - 2.930
R-factor	0.2071
Rwork	0.205
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yx2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.040
High resolution limit [Å]	2.930	2.930
Rmerge	0.100	0.538
Number of reflections	32961	1981
<I/σ(I)>	19.19
Completeness [%]	98.0
Redundancy	5.9
CC(1/2)		0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	2.7 M Sodium formate