6K60
Structural and functional basis for HLA-G isoform recognition of immune checkpoint receptor LILRBs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 164.378, 164.378, 326.839 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.806 - 3.149 |
| R-factor | 0.2628 |
| Rwork | 0.261 |
| R-free | 0.28860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2DYP and 3D2U |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.081 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.806 | 3.260 |
| High resolution limit [Å] | 3.149 | 3.150 |
| Rmerge | 0.100 | 0.501 |
| Number of reflections | 29650 | 2852 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M sodium iodide, 0.1M Bis Tris propane (pH 8.5), 20% PEG 3350 |
