6K1L
E53A mutant of a putative cystathionine gamma-lyase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.970, 148.460, 156.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.105 - 2.460 |
R-factor | 0.2084 |
Rwork | 0.206 |
R-free | 0.27570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nmp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.004 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.230 | 2.520 |
High resolution limit [Å] | 2.460 | 2.460 |
Number of reflections | 51467 | 3612 |
<I/σ(I)> | 11.4 | |
Completeness [%] | 99.3 | |
Redundancy | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Potassium sulphate, 20% (v/v) polyethylene glycol 3350, 5 mM cysteine |