6K0X

Structure of N6AMT1-TRMT112 Complex with SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	109.607, 109.607, 130.368
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.050 - 2.200
R-factor	0.1764
Rwork	0.174
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q87 4qtu
RMSD bond length	0.007
RMSD bond angle	0.917
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	24031	2346
<I/σ(I)>	28
Completeness [%]	99.8
Redundancy	36.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	0.2M ammonium acetate 0.1M sodium citrate tribasic dihydrate PH 5.6 30% Polyethylene glycol 4000