6K0R

Ruvbl1-Ruvbl2 with truncated domain II in complex with phosphorylated Cordycepin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	110.304, 186.424, 235.176
Unit cell angles	90.00, 91.04, 90.00

Refinement procedure

Resolution	49.720 - 2.502
R-factor	0.23
Rwork	0.228
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xsz
RMSD bond length	0.008
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	235.137	2.810
High resolution limit [Å]	2.502	2.502
Rmerge	0.107	1.033
Rmeas	0.120	1.138
Rpim	0.054	0.474
Number of reflections	90883	4542
<I/σ(I)>	9.1
Completeness [%]	89.9
Redundancy	4.6
CC(1/2)	0.996	0.159

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M HEPES-Na pH 7.5-7.6, 0.2 M MgCl2, 20-21 % PEG400