6JWI
Yeast Npl4 in complex with Lys48-linked diubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97904 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.351, 103.103, 99.620 |
Unit cell angles | 90.00, 100.40, 90.00 |
Refinement procedure
Resolution | 48.990 - 2.550 |
R-factor | 0.1924 |
Rwork | 0.191 |
R-free | 0.22570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jwh |
RMSD bond length | 0.010 |
RMSD bond angle | 1.215 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.580 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 55810 | 2546 |
<I/σ(I)> | 10.3 | |
Completeness [%] | 99.1 | 91.3 |
Redundancy | 19.3 | 6.1 |
CC(1/2) | 0.504 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM Bicine-NaOH (pH 6.5), 18% PEG 3350, 200mM lithium sulfate |