6JTG

Structural insights into G domain dimerization and pathogenic mutations of OPA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9785
Spacegroup name	P 43 21 2
Unit cell lengths	77.890, 77.890, 171.547
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.345 - 2.400
R-factor	0.2037
Rwork	0.199
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.104
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	21509
<I/σ(I)>	20.56
Completeness [%]	99.9
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M Potassium thiocyanate and 20%(w/v) Polyethylene glycol 3350 (pH 7.0)