6JTA
Crystal Structure of D464A L465A mutant of FGAM Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 65 |
| Unit cell lengths | 146.800, 146.800, 141.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.720 - 1.750 |
| R-factor | 0.15225 |
| Rwork | 0.151 |
| R-free | 0.18052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3t |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.292 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmeas | 0.069 | 0.483 |
| Number of reflections | 172853 | 99074 |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2M Ammonium Sulphate |
