6JPJ

Crystal structure of FGF401 in complex of FGFR4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-08-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5
Spacegroup name	P 1 21 1
Unit cell lengths	42.275, 60.931, 61.154
Unit cell angles	90.00, 98.56, 90.00

Refinement procedure

Resolution	41.800 - 2.638
R-factor	0.1978
Rwork	0.195
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jkg
RMSD bond length	0.003
RMSD bond angle	0.675
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	2.700
High resolution limit [Å]	2.638	2.638
Rmerge	0.080	0.180
Number of reflections	8724
<I/σ(I)>	14.36
Completeness [%]	95.3
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M MES pH 5.5, 20% PEG 4000, 0.15M (NH4)2SO4, 4% (v/v) formamide