6JJO
Crystal structure of the DegP dodecamer with a modulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 217.148, 123.518, 140.558 |
Unit cell angles | 90.00, 118.00, 90.00 |
Refinement procedure
Resolution | 35.610 - 4.157 |
R-factor | 0.2191 |
Rwork | 0.216 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3otp |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.270 |
High resolution limit [Å] | 4.200 | 11.360 | 4.200 |
Rmerge | 0.112 | 0.056 | 0.665 |
Rmeas | 0.121 | 0.061 | 0.718 |
Rpim | 0.045 | 0.024 | 0.269 |
Total number of observations | 176600 | ||
Number of reflections | 24531 | 1230 | 1227 |
<I/σ(I)> | 9.1 | ||
Completeness [%] | 99.8 | 96.9 | 100 |
Redundancy | 7.2 | 6.4 | 7.1 |
CC(1/2) | 0.995 | 0.921 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 11% PEG3350 and 0.1 M tacsimate pH 3.5 |