6J4Q

Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	P 32 2 1
Unit cell lengths	117.177, 117.177, 209.044
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.307 - 2.700
R-factor	0.1868
Rwork	0.183
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j4o
RMSD bond length	0.010
RMSD bond angle	1.192
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.090	1.767
Rpim	0.030	0.585
Number of reflections	51721	5049
<I/σ(I)>	25.6
Completeness [%]	100.0	100
Redundancy	9.9	10
CC(1/2)	0.991	0.621

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	V2c-SVBP was mixed with the inhibitor TPCK at a mole ratio of 1:5 and incubated on ice for 30 min. Then, the resulting co-complex of V2c-SVBP-TPCK was crystallized in 1.0 M sodium malonate, pH 7.0.