6IYB

Structure of human ORP1 ANK - Rab7 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-14
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	55.136, 117.222, 130.476
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.286 - 2.091
R-factor	0.1967
Rwork	0.195
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t91
RMSD bond length	0.008
RMSD bond angle	0.926
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.090	2.090
Rmerge	0.069	0.324
Number of reflections	50042	2496
<I/σ(I)>	39.3	7.3
Completeness [%]	99.1	99.9
Redundancy	5.7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	0.1 M Na3Citrate-HCl, 1 M LiCl, 20% PEG 1000