6ISU
Crystal structure of Lys27-linked di-ubiquitin in complex with its selective interacting protein UCHL3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2018-01-10 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.407, 94.204, 91.178 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.850 - 1.866 |
R-factor | 0.2121 |
Rwork | 0.209 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xd3 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.861 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.850 | 1.933 |
High resolution limit [Å] | 1.866 | 1.866 |
Number of reflections | 29404 | |
<I/σ(I)> | 31.32 | |
Completeness [%] | 88.8 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 18% PEG 3350 (w/v), 400 mM Ca(AC)2 |