6IJN
The D295N mutant of the N6-methyl-AMP deaminase from Arabidopsis thaliana complexed with N6m-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.912, 80.086, 86.792 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.268 - 1.660 |
R-factor | 0.1487 |
Rwork | 0.147 |
R-free | 0.17410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ada |
RMSD bond length | 0.009 |
RMSD bond angle | 1.065 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.720 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.103 | 0.471 |
Number of reflections | 42279 | 4139 |
<I/σ(I)> | 20.5 | 5.1 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 12.6 | 13 |
CC(1/2) | 0.998 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 32% PEG 3350, 0.1 M NaCl, 0.1 M Tris-HCl (pH 9.0). |