6IIW

Crystal structure of human UHRF1 PHD finger in complex with PAF15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 61 2 2
Unit cell lengths	36.716, 36.716, 220.147
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.691 - 1.699
R-factor	0.1771
Rwork	0.176
R-free	0.18880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3asl
RMSD bond length	0.005
RMSD bond angle	0.928
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.030	1.730
High resolution limit [Å]	1.699	1.700
Rmerge	0.065	0.497
Number of reflections	10762	538
<I/σ(I)>	27.5	5.6
Completeness [%]	100.0	99.9
Redundancy	17.2
CC(1/2)	0.999	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1 M HEPES (pH 7.5), 70% (v/v) MPD