6IHC

Crystal structure of (3R)-Hydroxyacyl-Acyl Carrier Protein Dehydratase(FabZ) Y100A mutant in complex with holo-ACP from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	185
Detector technology	IMAGE PLATE
Collection date	2016-09-07
Detector	MACSCIENCE DIP100S
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	272.528, 76.900, 72.991
Unit cell angles	90.00, 99.23, 90.00

Refinement procedure

Resolution	45.644 - 2.400
R-factor	0.1774
Rwork	0.175
R-free	0.21380
RMSD bond length	0.009
RMSD bond angle	0.975
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	56174
<I/σ(I)>	14.9
Completeness [%]	96.1
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% PEG 3350, 0.05M tri-Sodium citrate dehydrate