6IG2
Structure of mitochondrial CDP-DAG synthase Tam41 complexed with CTP, delta 74, F240A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 173.15 |
| Detector technology | PIXEL |
| Collection date | 2017-06-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 21 2 2 |
| Unit cell lengths | 55.266, 187.418, 186.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.604 - 2.882 |
| R-factor | 0.2185 |
| Rwork | 0.216 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ig4 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.477 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.880 | 2.900 |
| Rpim | 0.042 | 0.482 |
| Number of reflections | 44684 | 4338 |
| <I/σ(I)> | 20.7 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 289.15 | 0.1 M HEPES-Na, pH 7.5, 9% Tacsimate, pH 7.0, 2% PEG 3350, incubated with 10 mM MgCl2, 5 mM CTP for 1 h before crystallization |
