6ICV
Structure of SETD3 bound to SAH and unmodified actin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.186, 175.170, 66.499 |
Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
Resolution | 32.634 - 2.150 |
R-factor | 0.1695 |
Rwork | 0.168 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3smt |
RMSD bond length | 0.007 |
RMSD bond angle | 0.836 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_3092: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.135 | 0.609 |
Number of reflections | 74952 | 5708 |
<I/σ(I)> | 14 | |
Completeness [%] | 100.0 | |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M HEPES sodium pH 7.5 2% Polyethylene glycol 400 2.0M Ammonium sulfate |